%0 Journal Article
%T High mobility group box 1 protein (HMGB1) as an immune-modulating factor for polarization of human T lymphocytes
%A Lifeng Huang
%A Yongming Yao
%A Haidong Meng
%A Xiaodong Zhao
%A Ning Dong
%A Yan Yu
%A <br>Lifeng Huang
%A Yongming Yao
%A Haidong Meng
%A Xiaodong Zhao
%A Ning Dong
%A Yan Yu
%J 老年心脏病学杂志(英文版)
%D 2008
%I Science Press
%X Objective This study was performed to investigate the effect of high mobility group box-1 protein (HMGB 1) on immune function of human T lymphocytes in vitro and explore its potential role in cell-mediated immune dysfunction.Methods Fresh blood was obtained from healthy adult volunteers and peripheral blood mononuclear cells (PBMCs) were isolated,then rhHMGB 1 was added to PBMCs.Four-color flow cytometric (FCM) analysis was used for the measurement of intracellular cytokine including interleukin Results (1) Different stimulating time and dosage of rhHMGB 1 did not alter the number of IFN-a positive cells (Th 1).rhHMGB 1 stimulation provoked a dose-dependent and time-dependent increase in Th2 subset and decrease in ratio of Th 1 to Th2.(2) Compared with the untreated cells,when the cells were coincubated with rhHMGB 1 (10-100ng/ml) for 12 hrs,protein release of IL-2 and sIL-2R were significantly up-regulated.At 48 hrs,in contrast,protein production was relatively lower in cells after exposure to 100-1000 ng/ml rhHMGBI.Conclusions These findings demonstrated that HMGB1 has a dual influence on immune functions of human T lymphocytes.
%K High mobility group box-1 protein
%K Immunity
%K T lymphocytes
%K Th1/Th2<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=A9DB1C13C87CE289EA38239A9433C9DC&cid=AD36F34DDC3BA7B0&jid=4DC702B3A2386A4814E2FF9CFB799B27&aid=629CC842A085D2F87A8DCC56261CC5C1&yid=67289AFF6305E306&vid=94C357A881DFC066&iid=0B39A22176CE99FB&sid=771469D9D58C34FF&eid=339D79302DF62549&journal_id=1671-5411&journal_name=Journalofgeriatriccardiology:JGC&referenced_num=0&reference_num=19