%0 Journal Article
%T Following Enzyme Activity with Infrared Spectroscopy
%A Saroj Kumar
%A Andreas Barth
%J Sensors
%D 2010
%I MDPI AG
%R 10.3390/s100402626
%X Fourier transform infrared (FTIR) spectroscopy provides a direct, "on-line" monitor of enzymatic reactions. Measurement of enzymatic activity is based on the fact that the infrared spectra of reactants and products of an enzymatic reaction are usually different. Several examples are given using the enzymes pyruvate kinase, fumarase and alcohol dehydrogenase. The main advantage of the infrared method is that it observes the reaction of interest directly, i.e.,no activity assay is required to convert the progress of the reaction into an observable quantity.
%K vibrational spectroscopy
%K infrared spectroscopy
%K ATR
%K FTIR
%K enzyme activity
%U http://www.mdpi.com/1424-8220/10/4/2626