%0 Journal Article
%T Galvanostatic Entrapment of Penicillinase into Polytyramine Films and its Utilization for the Potentiometric Determination of Penicillin
%A Fatma Ismail
%A Samuel B. Adeloju
%J Sensors
%D 2010
%I MDPI AG
%R 10.3390/s100402851
%X A sensitive and reliable potentiometric biosensor for determination of penicillin has been developed by exploiting the self-limiting growth of the non-conducting polymer, polytyramine. Optimum polytyramine-penicillinase (PTy-PNCnase) films for potentiometric detection of penicillin were accomplished with monomer solutions which contained 0.03 M tyramine, 37 U/mL penicillinase, 0.01 M KNO3,and 3 mM penicillin with an applied current density of 0.8 mA/cm2 and an electropolymerisation time of 40 seconds. The potentiometric biosensor gave a linear concentration range of 3¨C283 ¦ÌM for penicillin and achieved a minimum detectable concentration of 0.3 ¦ÌM. The biosensor was successfully utilized for the detection of Amoxycillin and gave an average percentage recovery of 102 ¡À 6%. Satisfactory recoveries of penicillin G were also achieved in milk samples with the potentiometric biosensor when concentrations are ¡Ý20 ppm.
%K penicillin
%K penicillinase
%K polytyramine
%K biosensor
%K potentiometry
%U http://www.mdpi.com/1424-8220/10/4/2851