%0 Journal Article
%T Induction of macrophage inflammatory cytokines by Ox-LDL is ABCA1 dependent<br>
%A Zhi-Gang Guo
%A Jian-Hua Li
%A Di Xie
%A Wen-Yan Lai
%A Jia-Yi Wu
%A Ping-Sheng Wu
%A <br>
%J 老年心脏病学杂志(英文版)
%D 2010
%I Science Press
%X Objective The current study aimed to evaluate whether the induction of macrophage inflammatory cytokines by Ox-LDL is related to the expression of ABCA1 pathway. Methods After THP1/PMA macrophages were transfected with ABCA1 antisense oligonucleotides (100nmol/L) followed by treatment with Ox-LDL (30mg/L), the expressions of ABCA1, ICAM-1 and MCP-1 mRNA and protein were determined by real-time fluorescent quantitative RT-PCR, Western blot or ELISA. Results Ox-LDL induced expressions of ABCA1, ICAM-1, and MCP-1 at both mRNA and protein levels from THP1/PMA macrophages. Transfection with ABCA1 antisense oligonucleotides reduced ABCA1 mRNA levels after 3 and 6 hours and protein levels after 12 and 24 hours. The expression of ICAM-1 and MCP-1 induced by Ox-LDL was also decreased after inhibition of ABCA1 protein expression by ABCA1 antisense oligonucleotide decreased. Conclusion The induction of macrophage inflammatory cytokines by Ox-LDL is partially dependent on expression of ABCA1. Our studies disclose new functions of ABCA1 in macrophages (J Geriatr Cardiol 2010; 7:166-170).
%K ATP-Binding cassette A1
%K THP1/PMA macrophage
%K inflammatory cytokine<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=A9DB1C13C87CE289EA38239A9433C9DC&cid=AD36F34DDC3BA7B0&jid=4DC702B3A2386A4814E2FF9CFB799B27&aid=53D1930C2BB9CC71B5A4DFBEB5331585&yid=140ECF96957D60B2&vid=DF92D298D3FF1E6E&iid=E158A972A605785F&sid=43608FD2E15CD61B&eid=C5F8B8CB20F1B3D8&journal_id=1671-5411&journal_name=Journalofgeriatriccardiology:JGC&referenced_num=0&reference_num=0