%0 Journal Article
%T Evanescent Wave Fiber Optic Biosensor for Salmonella Detection in Food
%A Angela M. Valadez
%A Carlos A. Lana
%A Shu-I Tu
%A Mark T. Morgan
%A Arun K. Bhunia
%J Sensors
%D 2009
%I MDPI AG
%R 10.3390/s90705810
%X Salmonella enterica is a major food-borne pathogen of world-wide concern. Sensitive and rapid detection methods to assess product safety before retail distribution are highly desirable. Since Salmonella is most commonly associated with poultry products, an evanescent wave fiber-optic assay was developed to detect Salmonella in shell egg and chicken breast and data were compared with a time-resolved fluorescence (TRF) assay. Anti-Salmonella polyclonal antibody was immobilized onto the surface of an optical fiber using biotin-avidin interactions to capture Salmonella. Alexa Fluor 647-conjugated antibody (MAb 2F-11) was used as the reporter. Detection occurred when an evanescent wave from a laser (635 nm) excited the Alexa Fluor and the fluorescence was measured by a laser-spectrofluorometer at 710 nm. The biosensor was specific for Salmonella and the limit of detection was established to be 103 cfu/mL in pure culture and 104 cfu/mL with egg and chicken breast samples when spiked with 102 cfu/mL after 2¨C6 h of enrichment. The results indicate that the performance of the fiber-optic sensor is comparable to TRF, and can be completed in less than 8 h, providing an alternative to the current detection methods.
%K Salmonella
%K fiber optic sensor
%K time¨Cresolved fluorescence assay
%K egg
%K chicken
%U http://www.mdpi.com/1424-8220/9/7/5810