%0 Journal Article
%T Monitor and control of neuronal activities with femtosecond pulse laser
%A ZHOU Wei
%A LIU XiuLi
%A Lü
%A XiaoHua
%A LI JiaSong
%A LUO QingMing
%A ZENG ShaoQun
%A <br>
%J 科学通报(英文版)
%D 2008
%I 
%X Combined with the fluorescence labeling technique, two-photon microscopy excited with femtosecond pulse laser has become an important tool for neuroscience research. In this research, the calcium signals from neurons in rat cortex slice were monitored by a custom-built two-photon microscopy, and the spontaneous calcium signals and the pharmacological responses as well as the responses to femtosecond pulse laser stimulation were recorded. The results showed that the amplitude of the cal- cium signals increased in direct proportion to the corresponding electrical activities. Glutamate induced a calcium transient, but continuous application resulted in smaller response. Simultaneous monitoring of neuronal populations distinguished the neurons of different microcircuits. The femtosecond pulse laser induced local or global calcium signals in the pyramidal neurons. The approach of interrogation and control of neural activities using femtosecond pulse laser is non-contact, nondestructive, repeatable, and without any additional substrates, which will contribute to the development of neuroscience.
%K femtosecond pulse laser
%K two-photon excitation
%K neural activity
%K monitor and control<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=04B3F2C307B14B34682E50A9E8D34FBA&yid=67289AFF6305E306&vid=8E6AB9C3EBAAE921&iid=94C357A881DFC066&sid=F4C2D192FB73A21F&eid=D02611D1F8166C9A&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=0&reference_num=32