%0 Journal Article
%T Baculovirus-mediated expression of a Chinese scorpion neurotoxin improves insecticidal efficacy
%A FAN XiaoJun
%A ZHENG Bo
%A FU YueJun
%A SUN Yi &
%A LIANG AiHua
%A <br>
%J 科学通报(英文版)
%D 2008
%I 
%X An Buthus martensii Karsch Insect Toxin (BmK IT) gene was inserted into the genome of Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) to construct a recombinant baculovirus, AcMNPV-BmK IT. The expression of BmK IT was confirmed using RT-PCR, dot blot and SDS-PAGE analysis. Dose-lethal time responses to Spodoptera exigua larvae were compared between wild-type baculovirus AcMNPV and recombinant virus AcMNPV-BmK IT. At the concentration of 1 × 107 PlBs/mL, the median lethal time of recombinant baculovirus (LT50 = 73.6 h) on third instar S. exigua larvae showed an improvement of 13.2% over the efficacy of wild type virus (LT50 = 84.8 h) during a 192 h infection. Supported by the National Natural Science Foundation of China (Grant Nos. 30670282 and 30700534) and the Department of Science and Technology of Shanxi Province
%K scorpion neurotoxin
%K BmK IT
%K recombinant baculovirus
%K expression
%K bioassay<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=901A6B58ED71B497335F03D70A9794E3&yid=67289AFF6305E306&vid=8E6AB9C3EBAAE921&iid=59906B3B2830C2C5&sid=F0513F17AA41A8C6&eid=C7BC59D607A1E67B&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=3&reference_num=38