%0 Journal Article
%T Monitoring p21 mRNA expression in living cell based on molecular beacon fluorescence increasing rate
%A TANG HongXing
%A YANG XiaoHai
%A WANG KeMin
%A TAN WeiHong
%A LIU Bin
%A HE LiFang
%A WANG Wei
%A <br>TANG
%A HongXing
%A YANG
%A XiaoHai
%A WANG
%A KeMin
%A TAN
%A WeiHong
%A LIU
%A Bin
%A HE
%A LiFang
%A WANG
%A Wei
%J 科学通报(英文版)
%D 2008
%I 
%X Studying the expression level of mRNA in living cells will offer tremendous opportunities for ad-vancement in cell biology research,disease diagnostics,and drug discovery.In this paper,a molecular beacon(MB)specific for the important tumor suppressor gene p21 has been designed and synthesized.The fluorescence signal was detected in real-time after the MB entered the cytoplasm of nasopharyn-geal carcinoma cells.After injecting the p21MB into nasopharyngeal carcinoma cell and p33-trans-fected nasopharyngeal carcinoma cell,the consistent increase of fluorescent signal intensity was de-tected in both cell lines,and maximum fluorescence intensity achieved in about 15 min.In about 4 min following microinjection,the fluorescence increasing rate was significantly different between these two cell lines,which indicate the different p21 mRNA expression levels.The results obtained in the real-time detection were also validated by RT-PCR.Analysis of the initial fluorescence increasing rate can effi-ciently reduce the side effect of enzyme and improve the accuracy in living cell mRNA detection.
%K molecular beacon
%K p21 mRNA
%K nasopharyngeal carcinoma cell
%K living cell imaging
%K fluorescence increasing rate<br>p21
%K mRNA
%K 鼻咽癌
%K 癌细腻
%K 荧光增强率
%K 基因表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=3C4DEB7C19984EE8625F2C0278B71136&yid=67289AFF6305E306&vid=8E6AB9C3EBAAE921&iid=38B194292C032A66&sid=FA88DCCE84EA0A56&eid=3E3EF0DB5E6F2DA9&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=0&reference_num=27