%0 Journal Article
%T Comparative Study of Protein Immobilization Properties on Calixarene Monolayers
%A Hongxia Chen
%A Minsu Lee
%A Sungwook Choi
%A Jae-Ho Kim
%A Heung-Jin Choi
%A Sung-Hoon Kim
%A Jeabeom Lee
%A Kwangnak Koh
%J Sensors
%D 2007
%I MDPI AG
%R 10.3390/s7071091
%X Three calix[4]arene (Cal-4) derivatives of which contain ethylester (1),carboxylic acid (2), and crownether (3) at the lower rim with a common reactive thiol at theupper rim were synthesized and constructed to self-assembled monolayers (SAMs) on Aufilms. After spectroscopic characterization of monolayers, the interaction between Cal-4and surface confined bovine serum albumin (BSA) in the SAMs was analyzed by surfaceplasmon resonance (SPR). The estimated surface concentration of BSA on the Cal-4 SAMwith crownether group was the highest among the three Cal-4 derivatives. Anti-hIgG andhIgG pair was employed for the investigation of protein-protein interaction. Molecularinteraction between anti-hIgG and hIgG can be detected in a concentration range of 10pg/mL to 200 pg/mL on the Cal-4 derivative 3 SAM modified SPR chip.
%K Calix[4]arene
%K Self-assembled monolayer
%K Linker system
%K anti-hIgG
%K Biosensor.
%U http://www.mdpi.com/1424-8220/7/7/1091