%0 Journal Article
%T Effects of soy isoflavone extracts on the growth of estrogen-dependent human breast cancer (MCF-7) tumors implanted in ovariectomized nude mice<br>
%A WU Qian
%A JIN NianZu
%A YU Jing
%A ZHAO RenChen
%A YU ZePing
%A QIAO ShanLei
%A LU XiaoHe &|ZHANG ChunWen
%A <br>
%J 科学通报(英文版)
%D 2009
%I 
%X In this study, we explored the effects of soy isoflavone extracts on the growth of estrogen-dependent human breast cancer (MCF-7) tumors implanted in ovariectomized athymic mice. The ovariectomized athymic mice were implanted with MCF-7 cells. They were fed with low, moderate and high doses of soy isoflavone extracts, at dietary concentrations of 6.25, 12.5 and 25 g/kg, respectively. The expression of ki-67 was detected by immunohistochemistry. The pS 2 expression in tumors was analyzed by real-time PCR. Estrogen level in the serum was measured by chemiluminescence enzyme immunoassay. Compared with the control group, dietary soy isoflavone extracts had a significant stimulatory effect on MCF-7 tumor growth in mice (P < 0.05). The ki-67 and pS 2 mRNA expressions in tumors were significantly increased by 6.25 and 12.5 g/kg dose of soy isoflavone extracts (P < 0.05). And, estrogen level in serum of 6.25 and 12.5 g/kg dose groups was higher than that of control group (P > 0.05). In conclusion, in the tested dietary concentration range soy isoflavone extracts had a stimulatory effect on tumor growth. 6.25 and 12.5 g/kg doses of soy isoflavone extracts can increase the cell proliferation in tumors and induce estrogen-responsive pS2 expression. Supported by National Natural Science Foundation of China (Grant No. 30572133)
%K soy isoflavone extracts
%K breast cancer
%K nude mice
%K MCF-7
%K estrogen
%K ki-67
%K pS
%K 2<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=4BCF79C62F1CBBD404A4918BF4FA7919&yid=DE12191FBD62783C&vid=318E4CC20AED4940&iid=CA4FD0336C81A37A&sid=AA76E167F386B6B3&eid=E44E40A2398D4F2A&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=0&reference_num=34