%0 Journal Article
%T Functional maturation of two murine medullary-type CD8SP thymocytes<br>
%A Tian Tian
%A Xiaoping Qian
%A Ling Gao
%A Weifeng Chen
%A <br>
%J 科学通报(英文版)
%D 2001
%I 
%X TCRαβCD4-CD8+ thymocytes are heterogeneity. They may undergo phenotypic and functional maturation within thymic medulla. Medullary-type CD8SP thymocytes were divided into seven subsets based on phenotypic analysis, and their precursor-progeny relationship along with the differential pathway was also delineated. To further testify the validity of the maturation pathway, we purified 6C10-CD69+ cells representing the early stage and 6C10-Qa-2+ cells representing the later stage among medullary-type CD8SP thymocytes and compared their functional maturation levels. CD8+ T cells of spleen were used as the control. It is shown that there is no obvious difference of proliferation ability among these three subsets; however, intracytoplasmic cytokine assay shows that there is a hier archy of IFN-γ and TNFα secretion among these subsets, strikingly comparable to their phenotypic status among medullary type CD8SP thymocytes. The bioassays of IL-2 and IFN-γ in culture supernatant give the similar results.
%K CD4-CD8+ thymocyte
%K phenotype
%K function<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=CA35BDFE2142B1975A20A092FD1F6700&yid=14E7EF987E4155E6&vid=D997634CFE9B6321&iid=9CF7A0430CBB2DFD&sid=4C49EFA0A53C9DF0&eid=8A6D8366EA57F0FF&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=0&reference_num=0