%0 Journal Article
%T v-Fos transformation effector binds with CD2 cytoplasmic tail
%A LI Ming
%A ZHANG Weilun
%A LIU Shilian
%A LIU Yanxin
%A ZHENG Dexian
%A <br>LI
%A Ming
%A ZHANG
%A Weilun
%A LIU
%A Shilian
%A LIU
%A Yanxin
%A ZHENG
%A Dexian
%J 科学通报(英文版)
%D 2006
%I 
%X We previously reported that v-Fos transformation effector (Fte-1) is a novel CD2 binding protein identified by yeast two hybrid system. In the present study, we further characterize the molecular properties and biological activity of the Fte-1. In vitro interaction analysis by an IAsys Resonant Mirror Bio- sensor further demonstrated that Fte-1 interacts with CD2 specifically, and the dissociation constant (Kd) of Fte-1-CD2 is 10?7 mol. The molecular section analysis showed that protein kinase C (PKC) phos- phorylation site at Ser238 of Fte-1, as confirmed by in vitro phosphorylation, is essential for the specific in- teraction with CD2. Jurkat T cells transfected with expression vector encoding for EGFP-Fte-1 fusion protein showed that Fte-1 displays a clustering dis- tribution in the cells. Upon stimulation by CD2 mono- clonal antibody T11, Fte-1 lost the character of clus- tering distribution and translocation to the plasma membrane, which were disrupted by mutation of Fte-1 (Ser238Gly), suggesting that Fte-1 could be co-localized with CD2 on the membrane, and Fte-1 phosphorylation at Ser238 is a crucial factor in CD2-mediated interaction with Fte-1. Suppression of Fte-1 expression by small interference RNA (siRNA) diminished the susceptibility of Jurkat T cells to apoptosis triggered by phorbol 12-myristate 13-ace- tate (PMA) and ionomycin, indicating that the bio- logical function of Fte-1 may be involved in the regu- lation of activation and apoptosis of T cells. These results provide further evidence that Fte-1 is a novel binding protein of CD2, and may function as a down- stream molecule in the CD2-mediated events.
%K Fte-1
%K CD2
%K binding protein
%K PKC
%K phosphorylation<br>磷酸化
%K Fte-1
%K CD2
%K 结合蛋白
%K PKC
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=FD912C9F23662A9053CC297D39DF757B&yid=37904DC365DD7266&vid=987EDA49D8A7A635&iid=CA4FD0336C81A37A&sid=16D8618C6164A3ED&eid=F4B561950EE1D31A&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=0&reference_num=50