%0 Journal Article
%T Effects of antisense CENP-B on the proliferation of HeLa (Tet-off) cells<br>
%A LIANG Qianjin
%A WANG Qian
%A LIN Haiyan
%A LUO Song
%A WANG Chunmei
%A HE Dacheng
%A WANG Yongchao
%A <br>
%J 科学通报(英文版)
%D 2002
%I 
%X In order to study the change of the expression of centromere protein B (CENP-B) caused by antisense transfection, proto-eukaryotically expressed fused protein GST-CENP-B (65 ku) was injected into mouse, and a peculiar anti-CENP-B serum MaCenpB was collected. A strain of transfected HeLa Tet-off cell HaCb, which contains antisense CENP-B expressing vector pBI-EGFP-as-CenpB, was prepared. Northern blot and Western blot were used to analyze the repression of internal CENP-B in transfected cells. According to the growth curve, the proliferation of HeLa (Tet-off) is repressed by antisense CENP-B, and the multiplication time is prolonged for 32.81 h. The analysis of flow cytometry revealed that, compared with HeLa (Tet-off), the G1 cell population of HaCb is increased (ΔG1= 9%) while S fraction is decreased (ΔS = 11%), but the G2/M phase is nearly unchanged (ΔG2/M=3%). In the meanwhile, the mitotic index of HaCb declines greatly compared with that of HeLa (Tet-off). Immunofluorescence showed that the assembling of centromeres in HaCb cell is arrested. These results suggest that a normal expression of CENP-B may be necessary for cell proliferation.
%K centromere proteins
%K CENP-B
%K HeLa (Tet-off) cell<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=13D72B22210BF1E455635861933D49DE&yid=C3ACC247184A22C1&vid=F4B561950EE1D31A&iid=94C357A881DFC066&sid=9EF602EA28138BEA&eid=663FF78B2ADE7A2D&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=0&reference_num=18