%0 Journal Article
%T Characterization, development and exploitation of EST-derived microsatellites in Gossypium raimondii Ulbrich
%A Changbiao Wang
%A Wangzhen Guo
%A Caiping Cai
%A Tianzhen Zhang
%A <br>WANG
%A Changbiao
%A GUO
%A Wangzhen
%A CAI
%A Caiping
%A ZHANG
%A Tianzhen
%J 科学通报(英文版)
%D 2006
%I 
%X Microsatellite DNA or simple sequence repeats (SSRs) can be derived from expressed sequence tags (ESTs). These markers are important for gene mapping as well as marker-assisted selection (MAS). To develop EST-SSRs for cotton gene mapping, we selected and characterized functional markers in Gossypium raimondii, which consisted of 58906 non-redundant EST sequences from NCBI. Among them there were 2620 microsatellite sequences containing 2818 EST-SSRs, which amounted to 4.45% of the non-redundant starting sequence population. This incidence was equivalent to one EST-SSR in every 14.8 kb of G. raimondii genetic material. Among the different motifs ranging from 1 to 6 bp, trinucleotide repeats were most abundant (38.31%), followed by dinucleotide repeats (24.09%) and mononucleotide repeats (23.35%). Among all identified motif types, A/T had the highest frequency (18.67%), followed by AT/TA (14.83%). Among the compound motifs, tandem trinucleotides occurred with the highest frequency (48.65%). In all, we identified 1554 EST-SSRs primer pair sequences. 300 of them were randomly selected to screen the polymorphisms between the mapping parents G. hirsutum acc. TM-1 and G. barbadense cv. Hai7124, to construct linkage groups in cultivated allotetraploid cotton. Among them, 129 (43%) primer pairs were found to have polymorphisms. Using these EST-SSRs we can compare EST-SSR distributions among different cotton species and various chromosomal locations.
%K Gossypium raimondii Ulbrich
%K EST-SSRs
%K distribution
%K molecular marker
%K polymorphism<br>棉属
%K EST-SSRs
%K 微卫生DNA
%K 分子标记
%K 多态性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=520E65480963DF270EE8F29517E9D2A9&yid=37904DC365DD7266&vid=987EDA49D8A7A635&iid=94C357A881DFC066&sid=2E15A588990CC690&eid=B4F9D541F855CF96&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=7&reference_num=26