%0 Journal Article
%T Cloning, sequencing and structural analysis of a pea cDNA encoding EF-1α<br>
%A CONG Xiangyu
%A LING Shiyun
%A ZHU Yuxian
%A <br>
%J 科学通报(英文版)
%D 2000
%I 
%X A cDNA library with the capacity of 2.0 × 106 was constructed using mRNA extracted, from gibberellic acid (GA)-treated G2 pea seedlings and a 1.6 kb cDNA with 100 nucleotides of 5′ non-coding region and 223 nucleotides of 3′ non-coding region was obtained by random screening. The DNA fragment contains an open reading frame of 1 344 nucleotides and encodes 447 amino acids. Sequence analysis of DNA and deduced polypeptide demonstrated that it encoded for a pea elongation factor 1-alpha (EF-1α). The functional domains of EF-1α is well conserved and EF-1α can be a good candidate for studying molecular evolution.
%K pea cDNA library
%K EF-1α
%K sequence analysis<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=23B5BD305477732924F79B66AD88C9E0&yid=9806D0D4EAA9BED3&vid=94E7F66E6C42FA23&iid=E158A972A605785F&sid=302684F6FB4B24FF&eid=5957D6E0A50D26B5&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=1&reference_num=15