%0 Journal Article
%T Sequence analysis of mRNA polyadenylation signals of rice genes
%A Ying Lu
%A Chenxi Gao
%A Bin Han
%A <br>LU
%A Ying
%A GAO
%A Chenxi
%A HAN
%A Bin
%J 科学通报(英文版)
%D 2006
%I 
%X The formation of eukaryotic mRNAs involves the cleavage and polyadenylation of pre-mRNAs. To investigate the sequence requirement of putative polyadenylation signals (PASs), poly(A) sites and downstream elements (DUEs) in 3′-end-processing in rice, we compared expressed sequences tags (ESTs) with poly(A) extremity to full-length cDNA sequences and constructed a database of 12969 pre-mRNA sequences in  40–+40 nt surrounding the poly(A) sites, which were from 9953 genes. The alternative poly(A) sites were revealed in approximately 25% of mRNAs. Nearly 80% of pre-mRNAs showed stringent requirement of the YA (CA or UA) at poly (A) sites for polyadenylation. About 7.9% had the AAUAAA signals on  40– 1 nt upstream of the poly(A) sites. Over 60% of mRNAs probably used the one-or two-base variants of AAUAAA hexamers as their PASs in 3′ fragments. The single-base variants of AAUGAA revealed the high frequency in 11.5% of 3′ fragments. The DUEs were detected in 90% of pre-mRNAs, especially more than half of the pre-mRNAs with multi-base variants of AAUAAA had the DUEs surrounding the poly(A) site. The location of DUE is also important for defining the cleavage site. Although most of the rice pre-mRNAs did not contain AAUAAA signal, the existence of downstream elements ensured the efficiency of cleavage-polyadenylation
%K polyadenylation signals
%K U-rich downstream elements
%K cDNA
%K expressed sequences tags
%K RNA processing
%K rice<br>多聚腺苷酸
%K 序列分析
%K mRNA
%K 水稻
%K 基因工程
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=171554812F19F2F4C9DDD78675EB1242&yid=37904DC365DD7266&vid=987EDA49D8A7A635&iid=9CF7A0430CBB2DFD&sid=81A5772701933E75&eid=E6E6318AC4BCBFDB&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=0&reference_num=31