%0 Journal Article
%T A peptide fusion protein inhibits angiogenesis and tumor growth by blocking VEGF binding to KDR<br>
%A LEI Hetian
%A SHOU Chengchao
%A LIU Xiaoyin
%A HE Luowen
%A WU Jian
%A JIANG Beihai
%A LIU Meisheng
%A YANG Junshan
%A <br>
%J 科学通报(英文版)
%D 2002
%I 
%X Vascular endothelial growth factor (VEGF) binding to its tyrosine kinase receptors (KDR/FLK1, Flt-1) induces angiogenesis. In search of the peptides blocking VEGF binding to its receptor KDR/FLK1 to inhibit tumorangiogenesis and growth, we screened a phage display peptide library with KDR as target protein, and some candidate peptides were isolated. In this study, we cloned the DNA fragment coding the peptide K237 from the library, into a vector pQE42 to express fusion protein DHFR-K237 in E. coli M15. The affection of fusion protein DHFR-K237 on endothelial cell proliferation and angiogenesis was investigated. In vitro, DHFR-K237 could completely block VEGF binding to KDR and significantly inhibit the VEGF-mediated proliferation of the human vascular endothelial cells. In vivo, DHFR-K237 inhibited angiogenesis in chick embryo chorioallantoric membrane and tumor growth in nude mice. These results suggest that K237 is an effective antagonist of VEGF binding to KDR, and could be a potential agent for cancer biotherapy.
%K vascular endothelial cell growth factor (VEGF)
%K VEGFR
%K peptide
%K antiangiogenesis<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=CC5895BC5EACC4D5A6204CB2A81CBBEE&yid=C3ACC247184A22C1&vid=F4B561950EE1D31A&iid=5D311CA918CA9A03&sid=6D25DD85174CF6DB&eid=710C005323C0774A&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=0&reference_num=15