%0 Journal Article
%T PF18-3, a 35-ku molecule, expresses specifically on apoptotic subset of mouse thymocytes<br>
%A WANG Hong
%A LI Yan
%A PANG Xuewen
%A CHEN Weifeng
%A <br>
%J 科学通报(英文版)
%D 2000
%I 
%X PF18-3 monoclonal antibody (mAb), one of the rat mAbs against mouse thymic stromal cells (MTSC), has been found to inhibit thymocyte apoptosis induced by a mouse thymic dendritic cell line, MTSC4, in previous co-culture study. The aim of this research is to investigate the character of PF18-3 rnAb recognized molecule (PF18-3 molecule) and its role in MTSC4-induced thymocyte apoptosis. The characterization of PF18-3 molecule expression has been conducted by FACS analysis. PF18-3 molecules have been found to express on MTSC4 as well as on Con A activated but not freshly isolated thyrnocytes. Up-regulated expression of PF18-3 molecules has been also observed on thymocytes after being co-cultured with MTSC4 for 48 h. The results from FACS analyses by PI staining for detecting apoptosis-related hypodiploid and by PF18-3 rnAb staining reveal that PF18-3 molecules expresss specifically on the apoptotic subgroup of thymocytes with high hypodiploid content. The PF18-3 molecule expressed on apoptotic thymocytes with 35 ku of molecular weight, identified by immunoprecipitation and western blotting, is thus likely to be a molecule involved in thymocyte apoptosis.
%K thymocytes
%K cell apoptosis
%K monoclonal antibody
%K mediator<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=E7B27501EC681C65AA476660D41397E4&yid=9806D0D4EAA9BED3&vid=94E7F66E6C42FA23&iid=E158A972A605785F&sid=EF78DD85C21CB57F&eid=FA3423FC1AE95C4E&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=0&reference_num=10