%0 Journal Article
%T Negative cooperative effect of cytotoxicity of a di-component initiating system for a novel injectable tissue engineering hydrogel
%A Shifeng Duan
%A Wen Zhu
%A Lin Yu
%A Jiandong Ding
%A <br>DUANShifeng
%A ZHUWen
%A YULin
%A DINGJiandong
%J 科学通报(英文版)
%D 2005
%I 
%X Chemically cross-linked hydrogels constitute a novel injectable tissue engineering material. At present, one of the key problems is to find an appropriate initiator. This study evaluated the cytotoxicity in vitro of a water-soluble redox initiating system consisting of ammonium persulfate (APS) and N, N, N′, N′-tetramethylethylenediamine (TEM-ED). Gelation time of PEG diacrylate macromer in phosphate buffer saline solution was first adjusted to guarantee that the examined initiator concentrations are sufficiently high to trigger polymerization of macromers. NIH/3T3 fibroblasts were employed to examine cytotoxicity via MTT measurements and optical microscopic observations. It has been found that the combined APS/TEMED system exhibits negative cooperative effect, for the underlying cytotoxicity is even lower than that of APS or TEMED at certain concentrations.
%K tissue engineering
%K injectable biomaterial
%K hydrogel
%K ini- tiator
%K cytotoxicity<br>组织工程
%K 血管注射剂
%K 水凝胶
%K 初始系统
%K 细胞毒素
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=5F102834E950E4B697E97BFF1DFA02F1&yid=2DD7160C83D0ACED&vid=771152D1ADC1C0EB&iid=708DD6B15D2464E8&sid=8DEAC935CD342902&eid=BE1F29C193C78397&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=1&reference_num=15