%0 Journal Article
%T Tobacco curly shoot virus isolated in Yunnan is a distinct species of Begomovirus<br>
%A Yan Xie
%A Xueping Zhou
%A Zhongkai Zhang
%A Yijun Qi
%A <br>
%J 科学通报(英文版)
%D 2002
%I 
%X Virus isolate Y1 was obtained from tobacco showing curly shoot symptoms in Baoshan, Yunnan Province. Whitefly transmission test and virion morphology observation showed that it is a begomovirus. In reactions with 14 monoclonal antibodies raised against begomoviruses, Y1 was readily differentiated from begomoviruses reported in China, Pakistan and India. The complete nucleotide sequence of DNA-A was determined, it contains 2746 nucleotides, with two ORFs in virion-sense DNA and four ORFs in complementary-sense DNA. Comparisons with total DNA-A, intergenic region and deduced amino acid sequences of individual ORFs showed that Yl is a distinct Begomovirus species, for which the name Tobacco curly shoot virus (TCSV) is proposed. The total DNA-A of TCSV is most closely related to that of Tomato leaf curl virus from India (85% sequence identity). In contrast, the deduced coat protein of TCSV is most like that of Cotton leaf curl virus 72b isolate from Pakistan (98% amino acid sequence identity).
%K Tobacco curly shoot virus
%K Begomovirus
%K distinct species
%K recombination<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=5AD0B8A463F765A89369A3A7687A9433&yid=C3ACC247184A22C1&vid=F4B561950EE1D31A&iid=38B194292C032A66&sid=2BA123C6EB9D54C2&eid=1E41DF9426604740&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=2&reference_num=14