%0 Journal Article
%T Reverse micelles extraction ofnattokinase: From model system to real system<br>Reverse micelles extraction of nattokinase： From model system to real system
%A LIU Junguo
%A XING Jianmin
%A CHANG Tianshi
%A LIU Huizhou
%A <br>LIU
%A Junguo
%A XING
%A Jianmin
%A CHANG
%A Tianshi
%A LIU
%A Huizhou
%J 科学通报(英文版)
%D 2006
%I 
%X Nattokinase is a novel fibrinolytic en- zyme, which is homologous to Subtilisin Carlsberg. In this paper, Subtilisin Carlsberg was taken as a model protein of nattokinase. Effects of pH, ionic strength, concentration of isopropanol on the extraction of Subtilisin Carlsberg with AOT/isooctane reverse mi- celles system were investigated. Further, the process of reverse micelles extraction of nattokinase from fermentation broth was studied. By taking the reverse micelles of AOT/isooctane as extractant to perform a full extraction cycle, it was found that about eighty percent of the total activity of nattokinase in the fer- mentation broth could be recovered and the purifica- tion factor was about 2.5. Homologous protein could be reasonably used as model protein of a target protein.
%K reverse micelles extraction
%K nattokinase
%K Subtilisin Carlsberg
%K protein purification<br>反转胶束提取法
%K 枯草杆菌蛋白酶
%K 蛋白质净化
%K 模拟系统
%K 真实系统
%K 纳豆激酶
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=A3AD2676F7D54DB3905C53B17C32AA66&yid=37904DC365DD7266&vid=987EDA49D8A7A635&iid=DF92D298D3FF1E6E&sid=6920A1020E13BE87&eid=0018E43E61963A72&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=0&reference_num=13