%0 Journal Article
%T Crystal structure of thermostable catechol 2,3-dioxygenase determined by multiwavelength anomalous dispersion method<br>
%A JIANG Tao
%A JI Chaoneng
%A SHENG Xiaoyu
%A CHEN Mingqin
%A XIE Yi
%A GONG Weimin
%A MAO Yumin
%A <br>
%J 科学通报(英文版)
%D 2002
%I 
%X The selenomethionyl derivative of the thermostable catechol 2,3-dioxygenase (SeMet-TC23O) is expressed, purified and crystallized. By using multiwave length anomalous dispersion (MAD) phasing techniques, the crystal structure of TC23O at 0.3 nm resolutions is determined. TC23O is a homotetramer. Each monomer is composed of N-terminal and C-terminal domains (residues 1～153 and 153～319, respectively). The two domains are proximately symmetric by a non-crystallographic axis. Each domain contains two characteristic motifs which are found in almost all of extradial dioxygenases.
%K multiwavelength anomalous dispersion (MAD)
%K X-ray diffraction
%K thermostable catechol 2
%K 3-dioxygenase
%K crystal structure
%K synchrotron light source<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=028C30F9322AE18FD2E0610218F71EAD&yid=C3ACC247184A22C1&vid=F4B561950EE1D31A&iid=E158A972A605785F&sid=F416A9924F23B020&eid=F637763636425CAF&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=0&reference_num=16