%0 Journal Article
%T Archaeal acylamino acid releasing enzyme/lipase:Crystallization and preliminary crystallographic analysis in a new crystal form
%A Ganggang Wang
%A Renjun Gao
%A Haitao Yang
%A Shugui Cao
%A Yan Feng
%A Zihe Rao
%A <br>WANGGanggang
%A GAORenjun
%J 科学通报(英文版)
%D 2003
%I 
%X A primitive orthorhombic crystal form of acylamino acid releasing enzyme/lipase (APE1547) from hyperthermophilic archaeon Aeropyrum pernix strain K1 has been obtained at 291 K. The diffraction pattern of the crystal extends to 0.27 nm resolution at 100 K using Cu Ka radiation. The crystal belongs to the space group P212121 with unit cell dimensions of a = 6.399, b = 10.439 and c = 16.953 nm. The presence of two molecules per asymmetric unit gives a crys-tal volume per protein mass (Vm) of 0.0022 nm3 Da-1 and a solvent content of 43% by volume. A full set of X-ray dif-fraction data were collected to 0.3 nm from the native crys-tal.
%K AARE
%K Aeropyrum
%K pernix
%K orthorhombic crystal
%K asymmetric unit<br>古细胞
%K 脂肪酶
%K 结晶形成
%K 晶体结构分析
%K 酰基肽水解酶
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=A87F6DFB940EBBC26A4B7780F2391D7E&yid=D43C4A19B2EE3C0A&vid=B6DA1AC076E37400&iid=0B39A22176CE99FB&sid=E2546871E5B846EF&eid=3F0AF5EDBC960DB0&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=0&reference_num=20