%0 Journal Article
%T Analysis of components of conserved<br>Analysis of components of conserved “backbone sequences”among genomes of Shigella spp. strains
%A LIU Hong
%A PENG Junping
%A YANG Jian
%A SUN Lilian
%A CHEN Shuxia
%A Jin Qi
%A <br>LIUHong
%A PENGJunping
%A YANGJian
%A SUNLilian
%A CHENShuxia
%A JinQi
%J 科学通报(英文版)
%D 2004
%I 
%X Difference in the genomic compositions of pro-karyotes is the basis of the diversity in their biological characters. However, besides their flora- or strain-specific genes, those floras with closer relationship in the evolution also have conserved "backbone sequences", which reveal the marks of their origin and evolution, and these "backbone sequences" are just the basis of their elementary living abilities and common biological properties. Shigella is very closely related to E. coli in the origin and evolution, and may turn out to belong to the same genus. In this study, a microarray containing E. coli K-12 whole genome and Sf301 specific ORFs is used to investigate the genomic components of four Shigella strains. The results indicate that 16%-22% K-12 ORFs sequences are not detected in the genome of Shigella strains while the genome of Shigella contains at least 2800 conserved ORFs, which compose the common "backbone sequences". Advanced analysis indicated that the "backbone sequences" are the essential components in maintaining the normal physiological activities of intestinal bacteria. Furthermore, only 20% Sf301-specific ORFs exist in other strains simultaneously, which demonstrate the great genome heterogeneity and the genetic diversity among the strains.
%K Shigella spp
%K E
%K coli K-12
%K whole-genome microarray
%K backbone sequence<br>志贺氏菌
%K 原核生物
%K 生物学性质
%K 脱氧核糖核酸
%K DNA
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=6A3AF94B78E4236D23F9DC434502C504&yid=D0E58B75BFD8E51C&vid=2A3781E88AB1776F&iid=0B39A22176CE99FB&sid=04445C1D2BDA24EE&eid=1B97AE5098AEB49C&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=0&reference_num=0