%0 Journal Article
%T On the structure of AP-4 responsive element in the LTR of Jembrana disease virus
%A LIU Zijing
%A Yin Hongyan
%A DENG Gang
%A Yuan Yuhua
%A Wang Jinzhong
%A GENG Yunqi
%A CHEN Qimin
%A <br>LIU Zijing
%A YIN Hongyan
%A DENG Gang
%A YUAN Yuhua
%A WANG Jinzhong
%A GENG Yunqi & CHEN Qimin College of Life Sciences
%A Nankai University
%A Tianjin
%A China Correspondence should be addressed to Chen Qimin
%J 科学通报(英文版)
%D 2003
%I 
%X Previous studies with deletion and sequence analysis of JDV LTR showed that there is a putative AP-4 responsive element in LTR. By antisense transient assay and gel shifting assay, we for the first time demonstrated that AP-4 modulated JDV gene expression by binding DNA di-rectly to bovine cells. The results, derived from site-directed mutagenesis experiments, suggest that the six base pairs of AP-4 binding site (CAGCTG) have different effects on JDV gene expression. When the first two base pairs changed to GC, JDV gene expression is severely decreased.
%K JDV
%K cellular factor AP-4
%K trans-activator (Tat)
%K LTR
%K site-directed mutagenesis<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=A85FA78FBA82EF70BEC8CAD5748D2C5E&yid=D43C4A19B2EE3C0A&vid=B6DA1AC076E37400&iid=59906B3B2830C2C5&sid=8C8B0901DFA6ECA2&eid=49833082E2634437&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=0&reference_num=10