%0 Journal Article
%T Introduction of exogenous DNA into cotton via the pollen-tube pathway with GFP as a reporter<br>
%A Guocun Huang
%A Yuemei Dong
%A Jingsan Sun
%A <br>
%J 科学通报(英文版)
%D 1999
%I 
%X The green fluorescent protein (GFP) gene from the jellyfishAequorea victoria as a vital reporter for gene expression in plants is considered to have several advantages over other reporter genes. The pBIN35S-mGFP4 plasmid DNA has been introduced into cotton embryos by the pollen-tube pathway method. A transformed seedling has been verified according to its GFP-related fluorescence and Southern blotting analysis. The results provided direct and convincing facts in cytology and molecular biology for the pollen-tube pathway method, an efficient transformation technique used in plants.
%K green fluorescent protein (GFP)
%K pollen-tube pathway
%K cotton<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=19C86D4619F3879C7EE90FBB930B7DCF&yid=B914830F5B1D1078&vid=1AE5323881A5ECDC&iid=5D311CA918CA9A03&sid=6CDD207A90CE1EEC&eid=6CDD207A90CE1EEC&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=1&reference_num=11