%0 Journal Article
%T Two-dimensional crystallization and preliminary electron crystallographic result of partially purified F0 from porcine mitochondria<br>
%A ZHANG Xujia
%A SUN Jingchuan
%A YANG Hui
%A LI Huilin
%A HUANG Youguo
%A YANG Fuyu
%A <br>
%J 科学通报(英文版)
%D 2002
%I 
%X After removal of cytoplasmic sector F1 from submitochondrial particles of F0F1-ATP synthase complex with guanidine hydrochloride, the transmembrane sector F0 was specifically extracted from the stripped membranes in the presence of detergent CHAPS and partially purified. Two-dimensional crystals were produced by the reconstitution of the partially purified F0 into asolectin and microdialysis. The obtained crystals are able to diffract to 2 nm. The projection map of the negatively stained crystal shows that the crystal has p4212 symmetry, lattice constant, a=b=14.4 nm. A unit cell contains four F0 molecules.
%K membrane protein
%K proton channel F0
%K two-dimensional crystal
%K electron microscopy<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=B64F513FE6DA93A2A86BDA85907FAF83&yid=C3ACC247184A22C1&vid=F4B561950EE1D31A&iid=E158A972A605785F&sid=892C6E385D640C1E&eid=86C0C9A759FDA8CA&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=0&reference_num=12