%0 Journal Article
%T Adhesion of subsets of human blood mononuclear cells to porcine endothelial cells<br>
%A ZHANG Xiaofeng
%A FENG Zhimin
%A FENG Meifu
%A WANG Hongfang
%A Ban Liqin
%A <br>
%J 科学通报(英文版)
%D 2000
%I 
%X Cellular immune response is a major barrier to xenotransplantation, and cell adhesion is the first step in intercellular recognition. Flow-cytometric adhesion assay has been used to investigate the differential adhesions of monocyte (Mo), natural killer cell (NK) and T lymphocyte (T) present within human peripheral blood mononuclear cells (PBMC) to porcine aortic endothelial cells (PAEC), and to demonstrate the effect of human interferon-γ (hIFN-γ) or/and tumor necrosis factor-α (hTNF-α) pretreatment of PAEC on their adhesiveness for different PBMC subsets. The preferential sequence for PBMC subset binding to resting PAEC is Mo, NK and T cells, among which T cells show the slightest adherence; hTNF-α can act across the species, and augment Mo, NK and T cell adhesion ratios by 40%, 110% and 3 times, respectively. These results confirm at the cell level that host Mo and NK cells are major participants in the cellular xenograft rejection, thereby, providing a prerequisite for further studying the human Mo/NK-PAEC interactive mechanisms.
%K human peripheral blood mononuclear cell (PBMC)
%K porcine aortic endothelial cell (PAEC)
%K human tumor necrosis factor-α
%K (hTNF-α
%K )
%K cell adhesion
%K phenotypic analysis<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=C07943973912A5BBC4DC8DE87E13A13E&yid=9806D0D4EAA9BED3&vid=94E7F66E6C42FA23&iid=DF92D298D3FF1E6E&sid=C824C8F9F54AE9B9&eid=039DCCB9394D9766&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=0&reference_num=10