%0 Journal Article
%T Apoptosis in suspension culture of tobacco cells induced by heat shock<br>
%A Haoming Chen
%A Changhui Yan
%A Xiaofang Jiang
%A Yaoren Dai
%A <br>
%J 科学通报(英文版)
%D 1999
%I 
%X The induction of apoptosis in suspension culture of tobacco cells by heat shock is reported for the first time. Heat treatment (48° for 4 h) of tobacco cells led to the appearance of typical hallmarks of apoptosis. It was demonstrated by DNA laddering analysis that the cells treated with heat shock at 48°C for 4 h had a serious degradation of nuclear DNA into multi-nucleosomal sizes, suggesting that heat shock activated endogenous nuclease which led to DNA cleavage at the linkage sites between the nucleosomes, but ladders were very faint for DNA from2 and 9 h heat-treated cells. The terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labelling (TUNEL) detection also showed that most of these treated cells (48°C for4 h) displayed positive reactions, indicating a serious DNA 3′-OH cleavage in their nuclei. Moreover, some other cytological changes in apoptotic cells, such as cell shrinkage, chromatin aggregation, nucleus collapse, have also been observed by 4′, 6′-diamidino-2-phenyl-indole (DAPI) staining.
%K apoptosis
%K DNA ladder
%K TUNEL
%K Nicotiana tabacum<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=7AEB65BDEFDCE39D8B89A5A2A8CDEACE&yid=B914830F5B1D1078&vid=1AE5323881A5ECDC&iid=59906B3B2830C2C5&sid=C17A97755771D03C&eid=C17A97755771D03C&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=0&reference_num=12