%0 Journal Article
%T A new method of recovering polyhydroxyalkanoate from Azotobacter chroococcum<br>
%A DONG Zhaolin
%A SUN Xuenan
%A <br>
%J 科学通报(英文版)
%D 2000
%I 
%X After polyhydroxyalkanoate (PHA) fermentation for 42 –48 h by theAzotobacter chroococcum G-3, the PHA content reached more than 75% of the dry weight. Biomass was isolated from culture by centrifugation and pretreated with freezing to release PHA pellet, then was treated with 10 g/L sodium dodecyl sulfate (SDS) for 15 min to effectively solubilize lipid and protein. Subsequently, it was further purified by digesting with 30% sodium hypochlorite (NaClO) for 3 min to remove peptidoglycan and non-PHA biomass. Finally, 98% PHA was obtained by diluting and rinsing with water, and the PHA recovered was suitable for processing.
%K freezing treatment
%K separation
%K purification
%K polyhydroxyalkanoate (PHA)<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=25C9E83AF4B7F0A7&yid=9806D0D4EAA9BED3&vid=94E7F66E6C42FA23&iid=38B194292C032A66&sid=10828928EB89AD8E&eid=80BD0A2EF8664214&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=0&reference_num=11