%0 Journal Article
%T Molecular characterization of a K+ channel blocker in the scorpion Buthus martensii Karsch<br>
%A ZHU Shunyi
%A ZENG Xianchun
%A LI Wenxin
%A JIANG Dahe
%A <br>
%J 科学通报(英文版)
%D 2000
%I 
%X K+ channel blockers of scorpion venoms are of important value in studying pharmacology and physiology of specific K+ channel of cells. Based on the amino acid sequences of BmP01 previously characterized as a small-conductance Ca2+-activated K+ channel blocker, two “back to back” degenarate primers have been designed and synthesized for inverse PCR strategy, its full-length cDNA has been cloned from the venom gland of the Chinese scorpionButhus martensii. The cDNA is composed of 3 parts: 5′ UTR, ORF and 3′ UTR. The flanking sequence of translation initiation codon ATG is AAAATGA, which is highly conserved in scorpion Na+ channel toxin and protozoan genes, suggesting that these genes may have followed a common mechanism for translation initiation. The 3′ UTR contains poly(A) signal AATAAA. The open reading frame encodes a precursor of 57 residues with a signal peptide of 28 residues and a mature peptide of 29 residues. The signal peptide is rich in hydrophobic amino acid residues and its length is significantly different from that of the determined scorpion Na+ channel toxin. The deduced amino acid sequence of mature peptide is completely consistent with BmP01 previously determined by primary structure analysis.
%K Buthus martensii Karsch
%K K+ channel blocker cDNA
%K sequence analysis<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=6D9FFF6EAE09CC0ED2E7555DDD58045C&yid=9806D0D4EAA9BED3&vid=94E7F66E6C42FA23&iid=5D311CA918CA9A03&sid=547991B346A6585E&eid=81D76BB45305F8B7&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=0&reference_num=18