%0 Journal Article
%T Binding and endocytosis of monoterbium transferrin by K562 cells
%A Chungui Zhao
%A Binsheng Yang
%A Wei Zhang
%A Haijing Bai
%A Wen Liu
%A <br>ZHAO Chungui
%A YANG Binsheng
%A ZHANG Wei
%A BAI Haijing & LIU Wen . Institute of Biological Technology
%A Shanxi University
%A Taiyuan
%A China
%A . Institute of Molecular Science
%A Shanxi University
%A Taiyuan
%A China
%A . Chinese Institute of Radiation Protection
%A Taiyuan
%A China Correspondence should be addressed to Yang Binsheng
%J 科学通报(英文版)
%D 2003
%I 
%X Using isotopic labeling of human serum apotransferrin, the binding and the endocytosis of monoterbium transferrin (Tbc-apotransferrin, Tbc-apotransferrin-FeN) by K562 cells, a human leukemic cell line, have been investigated. There are about (8.58 ± 2.41) × 105 binding sites per cell surface at 0°C. The association constant for Tbc-apotransferrin binding is 4.1 × 10 mol 1· L, for Tbc-apotransferrin-FeN 2.7×107 mol 1 · L at 0°C. At pH 7.4, upon warming cells to 37°C, endocytosis starts. The rate constants for the endocytosis are about 0.97 min 1 and 0.31 min 1 and the endocytosis ratio reaches 56% and 80% for Tbc-apotransferrin and TbC-apotransferrin-FeN, respectively.
%K transferrin
%K Tb3+
%K 125I-labeling
%K K562 cells<br>K562细胞
%K 转铁蛋白
%K 铽离子
%K 结合
%K 内噬作用
%K 碘125标记
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=E5C659CFC22323EB01F4DAF9539BF5FA&yid=D43C4A19B2EE3C0A&vid=B6DA1AC076E37400&iid=0B39A22176CE99FB&sid=B344543C2864D684&eid=B47A0E731AF43EB2&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=0&reference_num=9