%0 Journal Article
%T Isolation and purification of BV I -2H from bee venom and analysis of its biological action<br>Isolation and purification of BVⅠ-2H from bee venom and analysis of its biological action
%A Wei Zhu
%A Benxiang Wang
%A Xun Zhu
%A <br>
%J 科学通报(英文版)
%D 2002
%I 
%X The medical use of bee venom for rheumatoid arthritis (RA) has a very long tradition. In this study, isolation and purification of polypeptides from bee venom were carried out on sephadex chromatography, heparin sepharose CL-6B chromatography and HPLC. Several fractions were extracted, and their effects on activation of splenocyte and THP-1 cell were studied. The inhibitory fraction was selected for further studies. Finally, BV I -2H that the HPLC elution profiles was a single peak was isolated by C8 column. ESIMS detection results showed that BV I -2H was a fraction of bee venom, and the molecular weight of the major component was 644.8. BV I -2H could inhibit ConA-induced splenocyte proliferation, IL-1 production and interfere with splenocyte cycle in mice. Moreover, BV I -2H could inhibit PMA-induced TNFα production in THP-1 cells, which was due to its inhibitory effects on TNFα mRNA expression and protein phosphorylation of IκBα. Our studies indicated that BV I -2H was one of the anti-inflammatory components of bee venom.
%K polypeptides of bee venom
%K splenocyte proliferation
%K cytokines
%K cell cycle
%K Iκ
%K Bα<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=AFB944C0423A66DD3E51060C9A9C0241&yid=C3ACC247184A22C1&vid=F4B561950EE1D31A&iid=708DD6B15D2464E8&sid=A02B0E6E62BE4F0C&eid=FE68044BBD4BDA5F&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=0&reference_num=21