%0 Journal Article
%T Regeneration of fertile plants from isolated tobacco zygotes by in vitro culture
%A Yuchi He
%A Mengxiang Sun
%A Hongyuan Yang
%A <br>HEYuchi
%A SUNMengxiang
%A YANGHongyuan
%J 科学通报(英文版)
%D 2004
%I 
%X Living zygotes of tobacco (Nicotiana Tabacum L.) SR-1 were isolated and cultured in vitro by the microcul-ture technique. Fertile plants were regenerated from the calli derived from cultured zygotes via organogenesis. Ovules were collected 120 h after pollination and used as feeder. MS combined with KM8p was selected as basic medium in the experiment. Zygotes isolated from ovules 108 h after pollina-tion turned out to be suitable material for in vitro culture. Over 80% such zygotes could divide and around 10% of them could grow into calli and regenerate fertile plants.
%K tobacco
%K zygote
%K in vitro culture
%K plant regeneration<br>烟草
%K 接合子
%K 微晶结构
%K 植物再生
%K 孢子体
%K 基因转换
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=D81CD2C648B2A0BDDAA5EDA5A9F2CE34&yid=D0E58B75BFD8E51C&vid=2A3781E88AB1776F&iid=5D311CA918CA9A03&sid=4A2C67480A6B9F95&eid=725C082EB6FD280F&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=3&reference_num=17