%0 Journal Article
%T Study the effects of divalent metallic ions on the combination of DNA and histones with fluorescence anisotropy assays
%A LIU YuYing
%A WANG PengYe DOU ShuoXing XIE Ping XI XuGuang
%A <br>LIU
%A YuYing
%A WANG
%A PengYe
%A DOU
%A ShuoXing
%A XIE
%A Ping
%A XI
%A XuGuang
%J 科学通报(英文版)
%D 2007
%I 
%X The effects of divalent ions(Mn2 ,Mg2 ,Ca2 ) on the interaction between DNA and histone are studied using a fluorescence anisotropy assay. Fluorescence anisotropies of DNA and DNA-histone in the presence of divalent ions(Mn2 ,Mg2 ,Ca2 ) are measured. The results indicate that histone reduces the fluorescence anisotropy of lambda DNA while the divalent ions(Mn2 ,Mg2 ,Ca2 ) significantly enhance the fluorescence anisotropy. Compared to the case of DNA incubated with histone alone,there are more histones binding to DNA when divalent ion,histone and DNA are incubated together. We also find that Mn2 makes the DNA-histone complexes more condensed than the other ions do.
%K DNA
%K histone
%K divalent metal cation
%K fluorescence anisotropy<br>二价金属离子
%K DNA
%K 组蛋白
%K 荧光各向异性度检验
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=E463ADB93278BC4B08A2EDA2D4D62AE1&yid=A732AF04DDA03BB3&vid=286FB2D22CF8D013&iid=9CF7A0430CBB2DFD&sid=811F346FF129CCCC&eid=D610BE6A5A0C75BF&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=0&reference_num=19