%0 Journal Article
%T B→O blood conversion
%A ZHANG Yangpei
%A YANG Jun
%A GAO Xin
%A Jia Yanjun
%A JI Shouping
%A GONG Feng
%A LIU Zepeng
%A REN Huiming
%A LI Subo
%A LAN Jiongcai
%A CAO Qiong
%A <br>ZGHANYangpei
%A LANJiongcai
%J 科学通报(英文版)
%D 2003
%I 
%X To maintain a constant supply of universal blood type, or group O red blood cells, has benefit in specialized transfusion condition. In this study, a-galactosidase cDNA was cloned from Catimor coffee bean grown in Hainan island, China, by the RT-PCR method. We have constructed a vector for a-galactosidase cDNA expression and transferred a-galactosidase cDNA into Pichia pastoris GS115 cells by elec-troporation. The recombinant a-galactosidase (r-a-GalE) was purified by cation ion exchange chromatography. After studying the biochemical characters of r-a-GalE, we have succeeded in converting human erythrocytes from group B to group O. The animal experiment showed that transfusion of enzymetically converted group O red blood cells (ECORBC) was perfectly safe.
%K 红细胞血型
%K B型
%K 血型转化
%K O型
%K 重组α-半乳糖苷酶
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=6305FF77986AEAAE285E8516C5F0682D&yid=D43C4A19B2EE3C0A&vid=B6DA1AC076E37400&iid=38B194292C032A66&sid=1A363081E1FF7014&eid=9F8C5EF901EA1E7E&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=6&reference_num=9