%0 Journal Article
%T Establishment of Jurkat tet-on cell line<br>
%A WANG Hongwei
%A ZHANG Jing
%A WU Ninghua
%A SHEN Yufei
%A <br>
%J 科学通报(英文版)
%D 2000
%I 
%X Tet-control system is developed to tightly control target gene expression in mammalian cells by using the regulatory elements of tetracycline-repressor of the transposor Tn10 fromE. coli. We have transfected reverse tetracycline-controlled transactivator gene (rtTA) into genome of Jurkat cells and established two Jurkat tet-on cell lines. Induction of luciferase reporter activity with doxycycline, a tetracycline derivative, is dose-dependent with a peak value of 32-fold increment. Establishment of Jurkat tet-on cell lines greatly facilitates quantitative studies on target gene functions in the cells.
%K tet-on cell line
%K Jurkat cell
%K tetracycline control system<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=5641A183FEACFC3049C5837810217A6C&yid=9806D0D4EAA9BED3&vid=94E7F66E6C42FA23&iid=B31275AF3241DB2D&sid=640CCB6E396307A8&eid=323E8A365B085E0B&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=0&reference_num=7