%0 Journal Article
%T Studies on the Interaction Mechanism of 1,10-Phenanthroline Cobalt(II) Complex with DNA and Preparation of Electrochemical DNA Biosensor
%A Shuyan Niu
%A Feng Li
%A Shusheng Zhang
%A Long Wang
%A Xuemei Li
%A Shiying Wang
%J Sensors
%D 2006
%I MDPI AG
%R 10.3390/s6101234
%X Fluorescence spectroscopy and ultraviolet (UV) spectroscopy techniques coupled with cyclic voltammetry (CV) were used to study the interaction between salmon sperm DNA and 1,10-Phenanthroline cobalt(II) complex, [Co(phen)2(Cl)(H2O)]ClĄ¤H2O, where phen = 1,10-phenanthroline. The interaction between [Co(phen)2(Cl)(H2O)]+ and double-strand DNA (dsDNA) was identified to be intercalative mode. An electrochemical DNA biosensor was developed by covalent immobilization of probe single-strand DNA (ssDNA) related to human immunodeficiency virus (HIV) on the activated glassy carbon electrode (GCE). With [Co(phen)2(Cl)(H2O)]+ being the novel electrochemical hybridization indicator, the selectivity of ssDNA-modified electrode was investigated and selective detection of complementary ssDNA was achieved using differential pulse voltammetry (DPV).
%K 1
%K 10-Phenanthroline
%K Cobalt(II)
%K Voltammetry
%K Intercalative
%K Electrochemical DNA biosensor
%U http://www.mdpi.com/1424-8220/6/10/1234