%0 Journal Article
%T Molecular verification of the integration of Tripsacum dactyloides DNA into wheat genome through wide hybridization
Microbionic and peristaltic robots in a pipe
%A QIU Jiwen
%A JIN Demin
%A LI Chuanyou
%A JIA Jianhang
%A OUYANG Ping
%A William TAI
%A WANG Bin
%A LI Dawei
%A
%J 科学通报(英文版)
%D 2000
%I
%X RAPD and RFLP analyses of double haploid lines which derived from hybridization between hexaploid wheat (Triticum aestivum L. 2n=42) and eastern gamagrass (Tripsacum dactyloides L.2n=4x=72) are reported. Two of the 340 Operon primers have been screened, which stably amplifiedTripsacum dactyloides (male parent) specific bands in the double haploid lines. These results confirm the fact thatTripsacum dactyloides DNA has been integrated into wheat genome by sexual hybridization at molecular level. This idea has been further testified by RFLP analysis. Application and potentials of transferringTripsacum dactyloides DNA into wheat genome by sexual hybridization in wheat breeding are discussed.
%K bread wheat
%K Tripsacum dactyloides
%K wide hybridization
%K RAPD
%K RFLP
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=C81D2943A000452F&yid=9806D0D4EAA9BED3&vid=94E7F66E6C42FA23&iid=B31275AF3241DB2D&sid=652E7E360EBE3082&eid=640CCB6E396307A8&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=0&reference_num=16