%0 Journal Article
%T Cloning and expression of ophc2,a new organphosphorus hydrolase gene<br>Cloning and expression of ophc2, a new organphosphorus hydrolase gene
%A WU Ningfeng
%A DENG Minjie
%A LIANG Guoyi
%A CHU Xiaoyu
%A YAO Bin & FAN Yunliu Biotechnology Research Institute
%A Chinese Academy of Agricultural Sciences
%A Beijing
%A China
%A <br>WUNingfeng
%A DENGMinjie
%A LIANGGuoyi
%A CHUXiaoyu
%A YAOBin
%A FANYunliu
%J 科学通报(英文版)
%D 2004
%I 
%X The amino acid sequences of N-terminal and internal peptide of OPHC2,purified from Pseudomonas pseudoalcaligenes strain C2-1 in our lab,are determined.The full-length organphosphorus hydrolase gene ophc2 is cloned by PCR using the degenerate primers designed according to the sequences and future inverse PCR.The ophc2 gene is 975 bp long with G+C content of 63%,comprising one open reading frame encoding a polypeptide of 324 amino acids with a molecular weight of 36 kD.The nucleotide sequence of ophc2 shows low homolo- gies with those organphosphorus hydrolase genes deposited in Gen- Bank,one of which exhibits the highest homology of 46.4% with ophc2.The organphosphorus hydrolase protein expressed in E.coli bears normal bioactivity.
%K Pseudomonas pseudoalcaligenes
%K organphospho-
%K rus hydrolase
%K gene cloning and expression<br>有机磷水解酶
%K 基因克隆
%K 基因表达
%K 假单胞菌拟等位基因
%K 氨基酸
%K ophc2
%K 环境污染
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=AB2FFD3F82C8F1B652CAACF9ACB8786A&yid=D0E58B75BFD8E51C&vid=2A3781E88AB1776F&iid=59906B3B2830C2C5&sid=076A4355FDA11BCF&eid=0522AC581E488FBF&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=0&reference_num=27