%0 Journal Article
%T Mitochondrial response and calcium ion change in apoptotic insect cells induced by SfaMNPV
%A XIU Meihong
%A PENG Jianxin & HONG Huazhu Key Laboratory of Pesticide & Chemical Biology
%A Ministry of Education
%A <br>XlUMeihong
%A PENGJianxin
%A HONGHuazhu
%J 科学通报(英文版)
%D 2005
%I 
%X Mitochondrial responses and changes of calcium ions in apoptotic insect SL-1 cells induced by Syngrapha falcifera multiple nuclear polyhedrosis virus (SfaMNPV) are reported in this paper. By using Rhodamine 123 as a fluorescent labeling probe, flow cytometry analysis and confocal laser scanning microscope observation we observed that the mitochondrial transmembrane potential (DYm) began to decrease in SL-1 cells at 4 h post infection and DYm reduced continuously with the extension of virus infection. Western blotting indicated that the Bcl-2 level in the mitochondria gradually declined and was down- regulated. Cells undergoing apoptosis were found to have an elevation of cytochrome c in the cytosol and a corresponding decrease in the mitochondria, which indicated that cytochrome c was released from mitochondria into cytosol. These results suggest that mitochondrion-mediated apoptotic signal transduction pathway exists in apoptotic insect cell induced by SfaMNPV. Cytosolic free calcium (Ca2+]i) concentration rapidly increased after SfaMNPV infection and the elevated calcium was tested to come partly from extracelllular cal-cium ion influx. Flow cytometry analysis indicated that the apoptosis in SL-1 cells was not influenced by established cytosolic calcium clamped conditions and the EGTA inhibit-ing calcium influx. Therefore, neither the elevation of cytoso-lic calcium ion nor extracellular calcium entry was the in-ducing factor of apoptosis, which hinted that the depletion of ER Ca2+ store contributed to SL-1 cell apoptosis induced by SfaMNPV.
%K baculovirus
%K Spodoptera litura cell line (SL-1)
%K apoptosis
%K mitochondria
%K Ca2+
%K cytochrome c<br>钙离子
%K 细胞排列
%K 线粒体
%K 细胞色素
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=9CF0A94F606E53FF9B3CA521E852BBE9&yid=2DD7160C83D0ACED&vid=771152D1ADC1C0EB&iid=59906B3B2830C2C5&sid=ACF317960D6DF955&eid=9978E8308B7F7CE6&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=0&reference_num=25