%0 Journal Article
%T Oligomerization study of NHR3 and NHR4 domains from ETO protein involved in t(8;21)-associated acute myeloid leukemia<br>Oligomerization study of NHR3 and NHR4 domains from ETO protein involved in t（8;21）-associated acute myeloid leukemia
%A Wu Donghui
%A Yang Haitao
%A Xue Xiaoyu
%A Liang Wenxue
%A Miao Xiaoyu
%A Chen Saijuan
%A Pang Hai
%A <br>WUDonghui
%A YANGHaitao
%A XUEXiaoyu
%A LIANGWenxue
%A MIAOXiaoyu
%A CHENSaijuan
%A PANGHai
%J 科学通报(英文版)
%D 2005
%I 
%X Little had been known about ETO protein until t(8;21) was found in 12%–15% of acute myeloid leukemia which resulted in AML1-ETO fusion protein. ETO protein has four conserved nervy homology regions termed NHR1-4. A lot have already been known about NHR 1,2,4: NHR1 is homologous with theDrosophila TATA-box-associated factor 110 (TAF110); NHR2 is a dimerization domain associated with mSin3A/HDAC; NHR4 is MYND class of zinc fingers associated with NCoR/SMRT/HDAC. Only the function of NHR3 remains unclear. In order to investigate whether NHR3 domain could participate in oligomerization, we cloned and purified this domain. Through gel filtration chromatography, dynamic light scattering and dissolved crystal electrophoresis, we found that NHR3 domain was a tight tetramer. Then we cloned NHR3+4 domain (i.e. NHR3 domain plus NHR4 domain), and discovered, by gel filtration chromatography and native PAGE, that NHR3+4 domain could form dimer in solution. This was the first time to observe that NHR3 and NHR4 domains may have some contribution to the oligomerization of ETO protein, which might recruit corepressors in the form of dimer, and stabilize ETO dimerization through convergent strength of NHR2, NHR3 and NHR4 domains and then stabilize corepressors recruitment. These speculations are very worthy of further evaluation.
%K NHR3 domain
%K NHR4 domain
%K tetramer
%K dimer
%K ETO
%K protein
%K AML1-ETO fusion protein<br>白血病
%K NHR3
%K NHR4
%K 发病机理
%K 治疗方法
%K 蛋白质
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=09DDC413326EC7AC7117B278EE5BE0DD&yid=2DD7160C83D0ACED&vid=771152D1ADC1C0EB&iid=9CF7A0430CBB2DFD&sid=4AA5FA7F666BDD0A&eid=D698D0190A84C2BD&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=1&reference_num=21