%0 Journal Article
%T Specific immune responses induced by a multi-epitope antigen of hepatitis C virus in mice and rabbits
%A HUANG Jiansheng
%A PENG Chunxian
%A XIE Yongmei
%A ZHANG Qian
%A SHEN Xianrong
%A JI Dongmei
%A Said A Saleh
%A JIA Fuxing
%A Kazunari K Yokoyama
%A XU Zhun
%A ZHENG Weiyang
%A LAN Hekui
%A GUO Mingqiu
%A REN Daming
%A <br>
%J 科学通报(英文版)
%D 2000
%I 
%X Five highly conserved and immunogenic epitopes of hepatitis C virus (HCV) have been chosen to form a multi-epitope antigen gene and fused with β-galactosidase gene to express a hybrid GZ-PCX antigen, which could be specifically recognized by human HCV sera. High level of anti-GZ-PCX IgG has been induced when mice or rabbits were immunized with GZ-PCX antigen emulsificated with complete Freund’s adjuvant or mixed with killed attenuatedSalmonella typhimurium SL3261. The specific anti-GZ-PCX IgG reached a high titer of 10-6, which remained for several months. Specific cytotoxic T lymphocyte (CTL) effects, delayed type hypersensitivity reaction (DTH) and proliferation of peripheral lymphocytes have been induced by GZ-PCX antigen or synthetic peptides. High level of anti-GZ-PCX slgG has been detected in mice’s intestinal washing fluids, which indicates that the antigen induced mucosal immunity as well as systematic immunity. The studies show that the HCV multi-epitope antigen induces high level of specific immune responses without obvious toxicity, which might be able to provide protectivity to any HCV genotypes and isolates.
%K multi-epitope antigen
%K vaccine
%K immune response
%K hepatitis C virus (HCV)<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=EE49E511633793DD&yid=9806D0D4EAA9BED3&vid=94E7F66E6C42FA23&iid=F3090AE9B60B7ED1&sid=6484E0C1B87D264C&eid=51FE5F6F4794AD16&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=0&reference_num=5