%0 Journal Article %T Isolation and analysis of SSEA-4 positive cells derived from fetal marrow mesenchymal stem cells %A LIU Daqing %A YANG Yinxiang %A GAO Yanhong %A YUAN Hongfeng %A QIN Lipeng %A WANG Yunfang %A NAN Xue %A SHI Shuangshuang %A YUE Wen %A PEI Xuetao %A
LIU %A Daqing %A YANG %A Yinxiang %A GAO %A Yanhong %A YUAN %A Hongfeng %A QIN %A Lipeng %A WANG %A Yunfang %A NAN %A Xue %A SHI %A Shuangshuang %A YUE %A Wen %A PEI %A Xuetao %J 科学通报(英文版) %D 2006 %I %X A big issue in stem cell research is to derive prospective totipotential stem cells. In this study, fMSC-SSEA-4 cells expressing SSEA-4 anti- gen were isolated from fetal marrow masenchymal stem cells (fMSCs) using immunomagnetic bead sorting technique. The totipotent cells were identified and their biological characteristics were further stud- ied. The expression of Oct-4 and SSEA-4, carcino- genicity, and the ability to differentiation of fMSC- SSEA-4 cells were evaluated to verify the totipotent potential. fMSC-SSEA-4 cells were isolated suc- cessfully from fMSCs (2.5% among fMSCs), while no obvious differences were seen in morphology, growth curve, cell cycle and immunophenotype, Oct-4 and SSEA-4 expression between fMSC-SSEA-4 cells and fMSCs. fMSC-SSEA-4 cells showed normal diploid chromosome karyotype and no carcinoma was in- duced after inoculation into nude mice. fMSC- SSEA-4 cells could be induced to fat cells, osteo- genic cells and neuron-like cells in vitro with different induced factors. The results indicated that there may be a few totipotent cells among the fMSCs and it may offer the experimental basis for the further study and application of fMSCs. %K fetal marrow mesenchymal stem cells (fMSCs) %K SSEA-4 antigen %K immunomagnetic bead sorting %K totipotency %K biolocigal char- acters
fMSCs %K SSEA-4抗原 %K 全能性 %K 干细胞 %K 分离 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=512B867EA94B819C374AAC4CADA44FCE&yid=37904DC365DD7266&vid=987EDA49D8A7A635&iid=59906B3B2830C2C5&sid=B60306E6036A044A&eid=819029E276C005ED&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=0&reference_num=16