%0 Journal Article
%T Isolation and analysis of SSEA-4 positive cells derived from fetal marrow mesenchymal stem cells
%A LIU Daqing
%A YANG Yinxiang
%A GAO Yanhong
%A YUAN Hongfeng
%A QIN Lipeng
%A WANG Yunfang
%A NAN Xue
%A SHI Shuangshuang
%A YUE Wen
%A PEI Xuetao
%A
LIU
%A Daqing
%A YANG
%A Yinxiang
%A GAO
%A Yanhong
%A YUAN
%A Hongfeng
%A QIN
%A Lipeng
%A WANG
%A Yunfang
%A NAN
%A Xue
%A SHI
%A Shuangshuang
%A YUE
%A Wen
%A PEI
%A Xuetao
%J 科学通报(英文版)
%D 2006
%I
%X A big issue in stem cell research is to derive prospective totipotential stem cells. In this study, fMSC-SSEA-4 cells expressing SSEA-4 anti- gen were isolated from fetal marrow masenchymal stem cells (fMSCs) using immunomagnetic bead sorting technique. The totipotent cells were identified and their biological characteristics were further stud- ied. The expression of Oct-4 and SSEA-4, carcino- genicity, and the ability to differentiation of fMSC- SSEA-4 cells were evaluated to verify the totipotent potential. fMSC-SSEA-4 cells were isolated suc- cessfully from fMSCs (2.5% among fMSCs), while no obvious differences were seen in morphology, growth curve, cell cycle and immunophenotype, Oct-4 and SSEA-4 expression between fMSC-SSEA-4 cells and fMSCs. fMSC-SSEA-4 cells showed normal diploid chromosome karyotype and no carcinoma was in- duced after inoculation into nude mice. fMSC- SSEA-4 cells could be induced to fat cells, osteo- genic cells and neuron-like cells in vitro with different induced factors. The results indicated that there may be a few totipotent cells among the fMSCs and it may offer the experimental basis for the further study and application of fMSCs.
%K fetal marrow mesenchymal stem cells (fMSCs)
%K SSEA-4 antigen
%K immunomagnetic bead sorting
%K totipotency
%K biolocigal char- acters
fMSCs
%K SSEA-4抗原
%K 全能性
%K 干细胞
%K 分离
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=512B867EA94B819C374AAC4CADA44FCE&yid=37904DC365DD7266&vid=987EDA49D8A7A635&iid=59906B3B2830C2C5&sid=B60306E6036A044A&eid=819029E276C005ED&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=0&reference_num=16