%0 Journal Article %T Construction of cDNA library from iron-deficiency induced maize roots and screening and identification of iron-stress gene Fdr3
%A YIN Liping %A QI Xiaoting %A LIU Xianglin %A HUO Chunyan %A BAI Yanxia %A QIU Zesheng %A ZHANG Fusuo %A
%J 科学通报(英文版) %D 2000 %I %X To isolate Fe-deficient related (Fdr) genes, an expression cDNA library of 4.5×105 pfu/μg has been constructed from maize roots in iron-stress. 6 clones have been screened from the cDNA library by differential hybridization screening. It is proved that anFdr3 cDNA clone expressed stronger under iron-deficient condition than under iron-sufficient one by Northern blot and Western blot. %K maize %K iron-deficient cDNA library %K differential hybridization screening %K iron-deficient related (fdr) clone
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=5407613B982A59479B08DEC4355143AD&yid=9806D0D4EAA9BED3&vid=94E7F66E6C42FA23&iid=59906B3B2830C2C5&sid=FB318F788ECEF1B2&eid=8FC26EAE44BDA92A&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=0&reference_num=12