%0 Journal Article
%T HLA-G inhibits xenogenetic cytotoxicity mediated by human NK cells and T lymphocytes against PECs<br>HLA－G inhibits xenogenetic cytotoxicity mediated by human NK cells and T lymphocytes against PECs
%A Bin Shi
%A Huijun Yin
%A Xiuying Huang
%A Fangzhen Sun
%A <br>SHIBin
%A YINHuijun等
%J 科学通报(英文版)
%D 2003
%I 
%X In order to investigate whether the non-classical HLA-G class I molecule protects the porcine endothelial cells (PECs) from the lysis mediated by human immune cells in pig to human discordant xenotransplantation, we have cloned HLA-G cDNA from a human placenta by RT-PCR. Mammalian expression vector, pEFG-neo, was constructed by insertion of HLA-G cDNA in pEF-neo. We obtained efficiently expressed PECs by stable transfection. Cytotoxicity assay showed that overexpression of HLA-G on PECs was sufficient to inhibit human NK-92 cell lysis. The level of lysis was equal to or less than that of the lysis of human umbilical vein endothelial cells mediated by human NK-92 cells. It also indicated that HLA-G inhibited the lysis of PECs mediated by xeno-antigen specific T lymphocytes. The reduction of lysis ranged between 59.1% and 88.9%. These findings suggest that the transgenic approach to overexpress HLA-G is believed to be a new immunotherapy in overcoming the immune rejections in xenotransplantion, including delayed xenograft rejection and cell-mediated rejection.
%K HLA-G
%K xenotrasplantation
%K immune rejection
%K gene overexpression
%K cytotoxicity<br>HLA-G
%K 异体移植
%K 免疫排斥
%K 基因过表达
%K 细胞毒性
%K 人类NK细胞
%K T淋巴细胞
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=FFD7ABB5A8A016391EED1455649F5BA7&yid=D43C4A19B2EE3C0A&vid=B6DA1AC076E37400&iid=0B39A22176CE99FB&sid=856C2E13D1000DB7&eid=D59111839E7C8BDF&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=0&reference_num=23