%0 Journal Article
%T BICP0 and its RING finger domain act as ubiquitin E3 ligases in vitro
%A Lirong Diao
%A Wentao Qiao
%A Qimin Chen
%A Chen Wang
%A Yunqi Geng
%A <br>DIAOLirong
%A QIAOWentao
%A CHENQimin
%A WANGChen
%A GENGYunqi
%J 科学通报(英文版)
%D 2005
%I 
%X Bovine infected-cell protein 0 (BICP0) encoded by bovine herpes virus 1 (BHV-1) immediate early gene is necessary for efficient productive infection, in a large part, because it activates all 3 classes of BHV-1 genes. It also has the ability to efficiently transactivate promoters that are not derived from BHV-1. To investigate the mechanism by which BICP0 achieves these effects, we expressed and purified BICP0 and its different mutants in E. coli. In vitro assays showed that both full-length BICP0 and its isolated RING finger domain induce the accumulation of polyubiquitin chains. Mutations within the RING finger region that abolish the in vitro ubiquitination activity also cause severe reduc- tions in BICP0 activity in other assays. Based on these, we conclude that BICP0 has the potential to act as an E3 ubiq- uitin ligase during viral infection and its RING finger do- main is necessary for this function. These strongly support the hypothesis that BICP0 might influence virus infection through its ability to interact with the ubiquitin-proteasome pathway.
%K BICP0
%K RING finger domain
%K ubiquitin (Ub)
%K ubiquitin
%K ligase E3<br>蛋白质
%K 编码
%K BICP0
%K 肺炎
%K 氨基酸
%K DNA
%K 基因
%K 神经细胞
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=00BA86F6FE5BEEF67AA0EC24A9841C21&yid=2DD7160C83D0ACED&vid=771152D1ADC1C0EB&iid=DF92D298D3FF1E6E&sid=9BF3B0483F192149&eid=DB7B2C790D19BE6E&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=0&reference_num=22