%0 Journal Article
%T Studies on the overexpression of the soybean GmNHX1 in Lotus corniculatus: The re-duced Na+ level is the basis of the increased salt tolerance<br>Studies on the overexpression of the soybean GmNHX1 in Lotus corniculatus： The reduced Na^＋ level is the basis of the increased salt tolerance
%A SUN Yanxiang
%A WANG Dan
%A BAI Yanling
%A WANG Ningning
%A WANG Yong
%A <br>SUN
%A Yanxiang
%A WANG
%A Dan
%A BAI
%A Yanling
%A WANG
%A Ningning
%A WANG
%A Yong
%J 科学通报(英文版)
%D 2006
%I 
%X The full length cDNA coding for a novel vacuolar Na+/H+ antiporter (GmNHX1) was cloned from soybean and determined to consist of 2591 bp with a 5′-untranslated region of 464 bp, an open reading frame (ORF) of 1641 bp, and a 3′-untranslated region of 486 bp. The deduced protein sequence contains 546 aa with the typical characters of the vacuolar Na+/H+ antiporters, and shares high similarity with that of AtNHX1, OsNHX1 and AgNHX1. The soybean genome showed a single copy of the GmNHX1 gene. Northern blot analysis demonstrated that the expression of the GmNHX1 was tissue-specific, increased by ABA treatment, NaCl, KCl, LiCl and dehydration stress, and lower in leaves but higher in roots and hypocotyls of salt-tolerant than salt-sensitive cultivars. The GmNHX1 was overexpressed under the control of a tandem cauliflower mosaic virus (CaMV) 35S promoter in the model leguminous plant Lotus corniculatus L. and conferred salt-tolerance of the transgenic plants. Measurements of Na+ and K+ contents in both roots and shoots demonstrated that the plantlets of GmNHX1-overexpressing lines had lower Na+ and K+ content, and higher K+/Na+ ratio than the controlled lines that were transformed with the empty vector, which indicates that the salt-tolerance conferred by GmNHX1 is closely related with decreased accumulation of Na+ in the transgenic plants.
%K soybean
%K GmNHX1
%K transformation of Lotus corniculatus L
%K salt tolerance<br>大豆
%K GmNHX1
%K 莲
%K 耐盐性
%K 盐胁迫
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=1BC0F8CFD05054A93246A20C4445F614&yid=37904DC365DD7266&vid=987EDA49D8A7A635&iid=708DD6B15D2464E8&sid=10F17081942653E7&eid=29AE54A8F28A81D3&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=1&reference_num=41