%0 Journal Article
%T Antimicrobial Peptides: New Recognition Molecules for Detecting Botulinum Toxins
%A Nadezhda V. Kulagina
%A George P. Anderson
%A Frances S. Ligler
%A Kara M. Shaffer
%A Chris Rowe Taitt
%J Sensors
%D 2007
%I MDPI AG
%R 10.3390/s7112808
%X Many organisms secrete antimicrobial peptides (AMPs) for protection againstharmful microbes. The present study describes detection of botulinum neurotoxoids A, Band E using AMPs as recognition elements in an array biosensor. While AMP affinitieswere similar to those for anti-botulinum antibodies, differences in binding patterns wereobserved and can potentially be used for identification of toxoid serotype. Furthermore,some AMPs also demonstrated superior detection sensitivity compared to antibodies: toxoidA could be detected at 3.5 LD50 of the active toxin in a 75-min assay, whereas toxoids B andE were detected at 14 and 80 LD50 for their respective toxins.
%K Antimicrobial peptide
%K botulinum toxin
%K detection
%K array
%K biosensor
%U http://www.mdpi.com/1424-8220/7/11/2808