%0 Journal Article
%T Construction of transposon-mediated baculovirus vector and expression of green fluorescent protein in insect cells and larvae<br>
%A Ying Zhu
%A Yipeng Qi
%A <br>
%J 科学通报(英文版)
%D 1999
%I 
%X A transposon-shuttle vector Hanpvid was constructed by using wild-type genomic DNA fromHeliothis armigera nuclear polyhedrosis virus (HaNPV). It could replicate inE. coli cells as a large plasmid and remain infectious when being induced into insect cells. Hanpvid comprises HaNPV DNA and a transposon cassette which includes a miniF replicon, a kanamycin resistance gene (kan), lacZa and an attachment site for Tn7 (attTn7). Recombinant virus rHa-FaGP was obtained after transposition of a donor plasmid carrying green fluorescent protein gene (gfp) and polyhedrin gene (ocu) into attTn7. SDS-PAGE analysis shows that both gfp and ocu genes were highly expressed inHeliothis armigera cells. Green Hemolymphocytes can be seen under a fluorescent microscope 4 d after recombinant virus rHa-FaGP infected the third-instar larvae. The infected larvae show strong green fluorescence 6 d post infection.
%K HaNPV
%K transposon-shuttle vector
%K recombinant virus
%K green fluorescent protein<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=7483CF1A378614E4609B3A5B2ACAB9A6&yid=B914830F5B1D1078&vid=1AE5323881A5ECDC&iid=0B39A22176CE99FB&sid=4C100B7696CE9E24&eid=4C100B7696CE9E24&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=2&reference_num=11